Contact

Eric Edward Bryant, PhD

Education

2011—2018: PhD, Columbia University
Department: GSAS Biological Sciences
Thesis: Systems genetics of DNA damage tolerance — cisplatin, RAD5 & CRISPR-mediated nonsense
Mentor: Rodney Rothstein
Co-Mentor: Alberto Ciccia
Committee: Songtao Jia, Elizabeth Miller & Matthew Weitzman
Keywords: Systems genetics / Genetic interaction networks / Landscape enrichment analysis / Synthetic lethality / DNA replication, recombination & repair / Chromosome mobility / CRISPR iSTOP / Bioinformatics / R statistical programming
2006—2009: BS, University of California Los Angeles
Department: UCLA Microbiology, Immunology & Molecular Genetics
2004—2006: West Valley College
Department: WVC Biology

2009—2011: Gene Oracle, Research Assistant
Role: De-novo gene synthesis & degenerate codon library construction. Pipeline management and development.
Manager: Shannon Phan

2019: screenmill R package: capture, annotate, quantify, review and analyze time-series colony growth. This package was used for colony quantification and interaction analysis in Bryant et al. 2019. A nice example analysis using screenmill can be found in Figure 1D.
2017: iSTOP R package: design guides to introduce stop codons with CRISPR-mediated base editors. This package was written to facilitate guide design for Billon et el. 2017. iSTOP can be configured to design guides to generate any desired missense mutation using any hypothetical base editor in any genome with annotated coding sequence coordinates.

2013: Columbia University, Teaching Assistant for Molecular Biology
Professors: Songtao Jia & Ron Prywes
2012: Columbia University, Teaching Assistant for Cell Biology
Professors: Elizabeth Miller & Chloë Bulinski

2018: Departmental distinction for PhD dissertation defense
2017—2018: TL1 NIH training grant, clinical and translational research
2016—2017: T32 NIH training grant, cancer biology
2013—2015: T32 NIH training grant, biological sciences
2013: James Howard McGregor award (student with unusual promise as a teacher of zoology)

2019-09-26: Rad5 dysregulation drives hyperactive recombination at replication forks resulting in cisplatin sensitivity and genome instability.
Bryant EE, Šunjevarić I, Berchowitz L, Rothstein R, Reid RJD.
Nucleic Acids Research. 2019 Sep 26;47(17):9144—9159
PMID: 31350889 – PMCID: PMC6753471 – DOI: 10.1093/nar/gkz631
2019-01-09: Systems genetics of DNA damage tolerance – Cisplatin, RAD5 & CRISPR-mediated nonsense.
Bryant EE.
Columbia University.
DOI: 10.7916/d8-k1d0-kb09
2017-09-21: CRISPR-mediated base editing enables efficient disruption of eukaryotic genes through induction of STOP codons.
Billon P*, Bryant EE*, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, and Ciccia A.
Molecular Cell. 2017 Sep 21;67(6):1068—1079.e4
PMID: 28890334 – PMCID: PMC5610906 – DOI: 10.1016/j.molcel.2017.08.008
^{*co-first authors}

2019-10-01: DNA damage triggers increased mobility of chromosomes in G1 phase cells.
Smith MJ, Bryant EE, Joseph FJ, Rothstein R.
Molecular Biology of the Cell. 2019 Oct 1;30(21):2620—2625
PMID: 31483739 – PMCID: PMC6761769 – DOI: 10.1091/mbc.E19-08-0469
2018-09-01: Increased chromosomal mobility after DNA damage is controlled by interactions between the recombination machinery and the checkpoint.
Smith MJ, Bryant EE, Rothstein R.
Genes & Development. 2018 Sep 1;32(17-18):1242—1251
PMID: 30181361 – PMCID: PMC6120718 – DOI: 10.1101/gad.317966.118
2016-10-01: A synthetic dosage lethal genetic interaction between CKS1B and PLK1 is conserved in yeast and human cancer cells.
Reid RJD, Du X, Šunjevarić I, Rayannavar V, Dittmar J, Bryant E, Maurer M, and Rothstein R.
Genetics. 2016 Oct 1;204(2):807—819
PMID: 27558135 – PMCID: PMC5068864 – DOI: 10.1534/genetics.116.190231